热带作物学报2011，Vol.32Issue(6)：1111-1115,5.DOI:10.3969/j.issn.1000-2561.2011.06.025
辣椒小G蛋白CaRab8基因全长cDNA的分离及表达特征的初步分析
Isolation and Primary Expression Analysis of a Full-Length cDNA Clone Encoding Small GTP-Binding Protein Gene CaRab8 in Pepper
摘要
Abstract
One 961bp full -length cDNA clone was isolated from pepper normalized cDNA library, which containing a complete 651 bp open reading frame and encoding a putative protein composed of 217 amino acids. Amino acid sequence deduced by this cDNA clone showed high similarity to NtRAB8-2 protein from tobacco. The full-length cDNA was named CaRab8. The protein encoded by this cDNA clone included conserved domains for guanine nucleotide binding and GTPase activities and five domains specific to Rab family including YYRGA domain. Real time quantitative PCR analysis showed the expression level of CaRab8 was induced by MeJA while was repressed by SA.关键词
辣椒/小G蛋白/实时定量RT-PCRKey words
Pepper/Small G protein/Real-time quantitative PCR分类
生物科学引用本文复制引用
赖燕,肖翔,林菁,虞露,陈桂信,官德义,何水林..辣椒小G蛋白CaRab8基因全长cDNA的分离及表达特征的初步分析[J].热带作物学报,2011,32(6):1111-1115,5.基金项目
国家自然基金(No.30600391、30971718)、高校博士点基金(No.20093515110004)、福建省自然基金重点项目(No.2008J0003)、省自然基金(No.2008J0049)、省创新人才项目(No.2007F3013).省科技重点项目(No.2008N0099). （No.30600391、30971718）
