中国海洋湖沼学报（英文版）2011，Vol.29Issue(5)：967-972,6.DOI:10.1007/s00343-011-0040-8
Genetic identification of two species of Pleuronichthys byDNA barcoding
Genetic identification of two species of Pleuronichthys byDNA barcoding
摘要
Abstract
DNA barcoding is a new method for biological taxonomy,offering the ability to identify species from fragments in any life-history stage.Pleuronichthys cornutus and P.japonicus are two morphologically similar species.Pleuronichthys japonicus has never been found previously in China.However,in this study,we identified both species using DNA barcoding (cytochrome c oxidase subunit I (COI)),the mtDNA control region and cytochrome b.The results reveal that:1) intraspecific variation in the DNA barcode is much less than interspecific variation; 2) the two morphologically similar species were placed into separate clades distinguishable by high bootstrap values; 3) COI barcodes are more powerful for identifying the two species than the other two mtDNA fragments.关键词
Pleuronichthys/DNA barcoding/COI/CR/Cyt bKey words
Pleuronichthys/DNA barcoding/COI/CR/Cyt b引用本文复制引用
ZHANG Hui,ZHANG Yan,GAO Tianxiang,LI Pengfei,XU Hanxiang..Genetic identification of two species of Pleuronichthys byDNA barcoding[J].中国海洋湖沼学报（英文版）,2011,29(5):967-972,6.基金项目
Supported by the Special Fund for Agro-scientific Research in the Public Interest (No.201003068) and Special Key Program of Zhejieng Provincial Department of Science and Technology (No.2008C 12011) （No.201003068）
