热带作物学报2011，Vol.32Issue(5)：854-860,7.DOI:10.3969/j.issn.1000-2561.2011.05.014
龙眼胚性愈伤组织ACC合成酶基因(DL-ACS1)的克隆及表达分析
Cloning and Expression of ACS Gene from Embryogenic Callus In Dimocarpus longan
摘要
Abstract
The cDNA of ACS (1-aminocyclopropane-l - carboxylate synthase), which was a rate- limiting enzyme of ethylene synthesis, was cloned with homologous cloning and RACE from embryogenic callus in Dimocarpus longan. The full length of DL-ACS cDNA (GenBank, FJ617537), about 1 817 bp, consisted of an ORF (open reading frame) of 1 437 bp , and 5' and 3' un-translated regions of 73 bp and 307 bp, respectively. The putative protein had 478 amino acids, and the identity to the other polypeptides varied between 77%~63%, and belonged to ACC Synthase family. The level of DL-ACS mRNA changed more dynamically at 8 different stages. The peak mRNA transcription level of DL -A CS gene occurred at the globular embryo stage, and the lowest mRNA transcription level of DL-ACS was at the toperdo embryo stage.关键词
龙眼/胚性愈伤组织/ACC合成酶基因/基因克隆/实时荧光定量PCRKey words
Dimocarpus longan/Embryogenic callus/Gene of 1-aminocyclopropane-l-carboxylate synthase/Gene cloning/Real-time reverse transcription PCR (q-PCR)分类
农业科技引用本文复制引用
李惠华,赖钟雄,苏明华,林玉玲..龙眼胚性愈伤组织ACC合成酶基因(DL-ACS1)的克隆及表达分析[J].热带作物学报,2011,32(5):854-860,7.基金项目
国家自然科学基金(31071787. 30471204)、福建省重大科技平台建设(2008N2001)资助项目的部分内容. （31071787. 30471204）
