热带作物学报2011，Vol.32Issue(5)：861-865,5.DOI:10.3969/j.issn.1000-2561.2011.05.015
2个番木瓜多聚半乳糖醛酸酶抑制蛋白基因的克隆与分析
Cloning and Analysis of Two Polygalacturonase Inhibiting Protein Genes from Papaya
摘要
Abstract
Two full-length polygalacturonase inhibiting protein (PGIP) cDNA and DNA were cloned by RT-PCR from papaya and the genes were named as CpPGIPl and CpPGlP2 respectively. The full length CpPGlPl was 984 bp which encoding a protein with 325 amino acids. The full length CpPGIP2 was 1 025 bp which coding for 326 amino acids. Sequencing analysis showed that the identical amino acid sequence of the two genes was 63.53%, while the identical nucleotide sequence was 66.54%, and no intron sequences were found in the two genes. The amino acid sequences of the two proteins both contained plant PGIP specific conserved leucine repeat sequence LXXLXXLXXLXLXXNXLXGXIPXX. The gene expression patterns in papaya fruit at different ripening stages were analyzed by semi-quantitative RT-PCR. The results showed that the expression patterns of the two genes basically showed similar trend. The expression of CpPGIP was the highest in green stage and decreased gradually with papaya fruit maturing.关键词
番木瓜/多聚半乳糖醛酸酶抑制蛋白(PGIP)/基因克隆/半定量RT-PCRKey words
Carica papaya L/Polygalacturonase inhibiting protein/Gene clone/Semi-quantitative RT-PCR分类
农业科技引用本文复制引用
申艳红,陈晓静,李荣荣,卢秉国,何玮毅..2个番木瓜多聚半乳糖醛酸酶抑制蛋白基因的克隆与分析[J].热带作物学报,2011,32(5):861-865,5.基金项目
福建农林大学校基金资助项日(08B03) （08B03）
福建省自然科学基金资助项目(2010J05048). （2010J05048）
