热带作物学报2011，Vol.32Issue(5)：870-873,4.DOI:10.3969/j.issn.1000-2561.2011.05.017
甘蔗叶片宿根矮化病的PCR检测
Detection of Leifsonia xyli subsp.xyli in Sugarcane Leaves of Different Growth Stages by Polymerase Chain Reaction
摘要
Abstract
PCR was used to detect Leifsonia xyli subsp. Xyli in sugarcane leaves collected from four growth stages (seedling stage, tillering stage, jointing stage and mature stage) , different position of the same plant and three portions of each leaf. The results indicated that leaves from all four growth stages could be diagnosed as positive for L xyli subsp. Xyli infection, In addition, leaves in different positions from L xyli subsp. Xyli positive plants were detected as positive as welL So leaf position didn't affect L xyli subsp. Xyli assay efficiency in the detected sugarcane cultivar.关键词
甘蔗/宿根矮化病/PCR/检测Key words
Sugarcane/Ratoon stunting disease/PCR/Detection分类
农业科技引用本文复制引用
赵婷婷,王俊刚,杨本鹏,冯翠莲,熊国如,蔡文伟,王文治,伍苏然,张树珍..甘蔗叶片宿根矮化病的PCR检测[J].热带作物学报,2011,32(5):870-873,4.基金项目
中央级公益性科研院所基本科研业务费(No.ITBBZD0724),现代农业产业技术体系建设专项基金(No.nycytx-24). （No.ITBBZD0724）
