热带作物学报2011，Vol.32Issue(6)：1097-1101,5.DOI:10.3969/j.issn.1000-2561.2011.06.022
剑麻根际联合固氮菌的分离及固氮活性测定
Isolation of Endophytic Diazotrophic Bacteria from Sisal and Determination of Their Nitrogenase Activity
摘要
Abstract
The associative nitrogen fixation bacteria in sisal rhizosphere, sampled from Changjiang County in Hainan Province, Fusui County in Guangxi Zhuang Autonomous Region and Leizhou City in Guangdong Province, were isolated and purified by Gas Chromatography on the NFM, CCM, Ashby and modified Dobereiner medium. And their nitrogen -fixing activity was measured by acetylene reduction method. The result indicated that, 50 bacterial strains had nitrogen -fixing activity. And a bacterial strain No. ASN004 with the highest nitrogenase activity of 1765.6590 nmol/(mL·h) was found and it had good exploitation potential.关键词
剑麻/根际/固氮菌/乙炔还原法Key words
Sisal/Rhizosphere/Nitrogen-fixing bacteria/Gas chromatography分类
农业科学引用本文复制引用
陈河龙,李庆洋,易克贤,高建明,郑金龙,刘巧莲,张世清..剑麻根际联合固氮菌的分离及固氮活性测定[J].热带作物学报,2011,32(6):1097-1101,5.基金项目
国家科技支撑计划(No.2007BAD48B06),中央级公益性科研院所基本科研业务费专项资金资助(No.lTBBZX0827),948项目(No.2010-Z6),海南省然科学基金(No.80510),国家麻类现代产业技术体系建设专项(No.CARS-19-E17). （No.2007BAD48B06）
