色谱2011，Vol.29Issue(8)：798-804,7.DOI:10.3724/SP.J.1123.2011.00798
加压毛细管电色谱-紫外检测法分析糖皮质激素及其在头发检测中的应用
Detection of glucocorticoids by pressurized capillary electrochromatography with ultra-violet detection and its application in hair analysis
摘要
Abstract
An effective and convenient method for glucocorticoid analysis in hair by reversed-phase pressurized capillary electrochromatography (pCEC) with ultra-violet (UV) detection was developed. Eight glucocorticoids (betamethasone, dexamethasone, prednisone, predniso-lone, prednisolone acetate, hydrocortisone acetate, cortisone acetate and corticosterone) were separated within 20 min in an isocratic mode pCEC. The separations were performed on a reversed-phase C18 column, and with 245 nm as the UV detection wavelength. The mobile phase was composed of 1. 5 mmol/L Tris buffer (pH 8.0) and acetonitrile (65:35, v/v), at a pump flow rate of 0.05 mL/min. All of the compounds showed good linearity in the range of 0.036 -4.0 mmol/L. The limits of detection (LODs) for all glucocorticoids were of μg/g levels. The proposed method was applied to the analysis of hair samples. The interference of hair matrices was effectively eliminated by protein enzymatic digestion, followed by a methanol extraction and a solid phase extraction (SPE) clean up step. The average recoveries of 71% - 85% at different fortified levels of glucocorticoids were achieved. This non-invasive method is useful for rapidly estimating the level of drug exposure in drug chronic abuse and monitoring the compliance of therapeutic drugs.关键词
加压毛细管电色谱/紫外检测/糖皮质激素/头发Key words
pressurized capillary electrochromatography ( pCEC) / ultra-violet ( UV) detection/ glucocorticoids/ hair分类
化学化工引用本文复制引用
李博祥,郑敏敏,卢兰香,吴晓苹..加压毛细管电色谱-紫外检测法分析糖皮质激素及其在头发检测中的应用[J].色谱,2011,29(8):798-804,7.基金项目
国家自然科学基金项目(21075017) （21075017）
