中国农业科学2011，Vol.44Issue(17)：3529-3536,8.DOI:10.3864/j.issn.0578-1752.2011.17.005
氮肥和多效唑对小麦茎秆木质素合成的影响及其与抗倒伏性的关系
Effects of Nitrogen and PP333 Application on the Lignin Synthesis of Stem in Relation to Lodging Resistance of Wheat
摘要
Abstract
[Objective] This study was carried out to elucidate the effects of nitrogen and PP333 application on lignin content in culm of wheat, and discuss the physiological mechanism of nitrogen and PP333 in regulating the lodging resistance of wheat,[Method] A field experiment was conducted with two nitrogen application rates and two PP333 levels to study the changes of snapping resistance, culm lodging resistance index (CLRI), lignin content and activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), cinnamyl alcohol dehydrogenase (CAD) and 4-coumarate CoA ligase (4CL) of wheat, and the effect of nitrogen and spraying PP333 on the lignin content, related enzyme activities in the lignin biosynthesis of wheat and their relationships with lodging resistance. [Result] Compared with the low N (225 kg·hm-2) rate, the high N (300 kg·hm-2) rate reduced the PAL, TAL, CAD and 4CL activities and lignin content, reduced snapping resistance and CLRI of the same cultivar. The application of PP333 improved the PAL, TAL and CAD activities, increased lignin content, snapping resistance and CLRI, thus reduced lodging area and lodging degree. Correlation analysis showed that the lignin content of basal stem had a significant and positive correlation with PAL, CAL and CAD activity and CLRI, respectively, yet had no significant correlation with 4CL activity.[Conclusion] The high N(300 kg·hm-2) rate reduced the PAL, TAL.CAD enzyme activities and lodging resistance. Spraying PP333 improved the PAL, TAL, CAD enzyme activities and lignin content, and thus enhanced the lodging resistance.关键词
小麦/木质素/酶活性/抗倒伏指数Key words
wheat/ lignin/ enzyme activity/ culm lodging resistance index(CLRI)引用本文复制引用
陈晓光,石玉华,王成雨,尹燕枰,宁堂原,史春余,李勇,王振林..氮肥和多效唑对小麦茎秆木质素合成的影响及其与抗倒伏性的关系[J].中国农业科学,2011,44(17):3529-3536,8.基金项目
国家“973”计划(2009CB 118602)、国家公益性行业(农业)科研专项(200803037) （2009CB 118602）
