中国农业科学2011，Vol.44Issue(17)：3603-3612,10.DOI:10.3864/j.issn.0578-1752.2011.17.013
草莓EST-SSR标记开发及在品种遗传多样性分析中的应用
Development of EST-Derived SSR Markers and Their Application in Strawberry Genetic Diversity Analysis
摘要
Abstract
[Objective] The objective of this study was to analyze the SSR distribution in strawberry ESTs and to develop new EST-derived SSR markers, and application of EST-SSR markers in strawberry genetic diversity analysis was also validated. [Method] A total of 55 750 EST sequences of strawberry were obtained from NCBI. These sequences were screened by using MISA software to search for SSR motifs. Sixty pairs of primers were designed by the software Primer3.0 Plus. The PCR products of these primers were detected by PAGE and some of them were recovered for sequencing. [Result] A total of 1 490 SSRs were identified from 1 334 strawberry EST sequences. The dinucleotide, trinucleotide and hexanucleotide repeats were the dominant types with the frequency of 36.17%, 26.51% and 19.87%, respectively. Among the 60 EST-SSR primers, 42 amplified distinct bands and expected products, and 36 were polymorphic. Eleven polymorphic EST-SSR amplicons were validated by resequencing the PCR products. A dendrogram tree generated by the 11 validated polymorphic EST-SSR markers revealed reasonable genetic relationships among 20 strawberry cultivars. [Conclusion] It is an efficient and economic way to develop SSR markers from the strawberry EST sequences, and the EST-SSR markers can be used in strawberry genetic analysis.关键词
草莓/EST/SSR/遗传多样性Key words
strawberry/ EST/ SSR/ genetic diversity引用本文复制引用
董清华,王西成,赵密珍,宋长年,葛安静,王静..草莓EST-SSR标记开发及在品种遗传多样性分析中的应用[J].中国农业科学,2011,44(17):3603-3612,10.基金项目
农业部保种专项项目 (NB2010-2130135-7)、北京市教委科技发展计划项目(KM200710020012) （NB2010-2130135-7）
