中国农业科学2011，Vol.44Issue(17)：3613-3621,9.DOI:10.3864/j.issn.0578-1752.2011.17.014
营养限制及补偿对羔羊小肠黏膜生长发育的影响
Effect of Undernutrition and Compensation on the Growth of Small Intestinal Mucosa in Lambs
摘要
Abstract
[Objective] The effects of undernutrition (0-60 d) and compensation (60-150 d) on live weight (BW) and development of small intestinal mucosa in lambs were investigated. [Method] Eighty Wu Zhu Mu Qin lambs at average live weight (14.72 ± 1.10) kg, were divided into control group (CG), restricted group 1 (RG1), restricted group 2 (RG2) and restricted group 3 (RG3). Dietary nutrition level were (ME: 10.61 MJ·kg-1, CP: 150.8 g·kg-1), (ME: 10.49 MJ·kg-1, CP: 92.4 g·kg-1), (ME: 8.36 MJ·kg-1, CP: 150.1 g·kg-1) and (ME: 8.10 MJ·kg-1, CP: 62.7 g·kg-1) during undernutrition period for CG, RG1, RG2 and RG3, respectively. However, in nutritional compensation phase all groups were offered the same levels diet (ME: 9.77 MJ·kg-1, CP: 124.8 g·kg-1). Weight of lambs was measured every week. Four lambs of each group were slaughtered at 0 d (at the beginning of undernutrition period), 60 d (at the end of undernutrition period) and 150 d (the end of nutrition compensation period). The samples of duodenum, jejunum and mid-ileum were taken and fixed by using paraformaldehyde phosphate buffer. After HE staining, morphological structure of intestinal mucosa were observed under light microscope. [Result] The results showed that at the end of nutrition restriction period, the average live weight of RG1, RG2 (P<0.05) and RG3 (P<0.01) was significantly lower than that of CG However, during the nutrition compensation period, the growth rate of RG3 was significantly higher than that of CG (P<0.05). Development and growth of the small intestine mucosa were affected by nutrition level of diets. The energy restricted group (RG2) showed better compensatory potency than that of the protein restricted group (RG1). [ Conclusion ] The results indicated that after restriction by different levels of energy and protein all lambs showed compensatory effect in live weight and small intestinal mucosa growth. The development of small intestine mucosa was more prone to be influenced by dietary protein content other than energy concentration. After 60 d restriction by the nutrition level of ME: 8.36 MJ·kg-1, CP: 150.1 g·kg-1, the development of small intestinal mucosa of RG2 reached complete compensation at the end of nutrition compensation period, and nutrients absorption and growth rate also increased correspondingly.关键词
营养限制/营养补偿/羔羊/小肠/黏膜结构/体重Key words
undernutrition/ compensation/ lamb/ small intestine/ mucosal structure/ body weight引用本文复制引用
刘小刚,李大彪,侯先志,考桂兰,王海荣,杨金丽,张崇志,夏巍..营养限制及补偿对羔羊小肠黏膜生长发育的影响[J].中国农业科学,2011,44(17):3613-3621,9.基金项目
国家自然科学基金项目 (30860194) （30860194）
