中国康复理论与实践2011，Vol.17Issue(10)：973-976,4.
针康法对局灶性脑缺血大鼠前肢运动功能及缺血区突触素和生长相关蛋白-43表达的影响
Effects of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Forelimb Motor Function and Expression of Svnaptophvsin and Growth-associated Protein-43 in Rats after Focal Cerebral Ischemia
摘要
Abstract
Objective To explore the effects of cluster needling of scalp acupuncture combined with rehabilitation (Tang's Approach) on forelimb motor function and expression of synaptophysin and growth-associated protein (GAP-43) in rats after focal cerebral ischemia. Methods Endothelin-l(ET-l) was used to induce focal cerebral ischemia model in rats with forelimb dysfunction. The rats were randomly divided into 5 groups: sham operation group, model group, rehabilitation group, acupuncture group, and acupuncture with rehabilitation group, each group was divided into 4 subgroups by 3 d, 7 d, 14 d, and 21 d. 24 hours after modeling, the cluster needle of scalp point with skilled forelimb reaching was trained in the acupuncture with rehabilitation group, skilled forelimb reaching was trained in the rehabilitation group, and the cluster needle of scalp point only were punctured and stimulated in the acupuncture group. But the model group had no intervention. The success rates of forelimb reaching were analyzed and immuno-histochemical technique was used to observe the expression of synaptophysin and GAP-43 in cortical ischaemic lesions of the rats in each group at different time points. Results At different time points after the treatment, the successful rate of forelimb reaching in the acupuncture with rehabilitation group was higher than the model group, rehabilitation group, and acupuncture group (P<0.05). Conclusion The cluster needling of scalp acupuncture combined with rehabilitation can promote recovery of forelimb motor function in rats after focal cerebral ischemia, which may associate with the expression of synaptophysin and GAP-43 in cortex surrounding following focal cortical ischaemic lesions.关键词
针康法/脑缺血/前肢运动功能/突触素/生长相关蛋白-43/大鼠Key words
Tang's Approach/ cerebral ischemia/ forelimb motor function/ synaptophysin/ growth-associated protein-43/ rats分类
医药卫生引用本文复制引用
唐强,刘宏光,王艳,朱路文,李洪梅..针康法对局灶性脑缺血大鼠前肢运动功能及缺血区突触素和生长相关蛋白-43表达的影响[J].中国康复理论与实践,2011,17(10):973-976,4.基金项目
哈尔滨市科技创新人才研究专项资金项目(No.2011RFXYS072). （No.2011RFXYS072）
