浙江大学学报（理学版）2011，Vol.38Issue(5)：525-529,5.DOI:10.3785/j.issn.1008-9497.2011.05.010
等温滴定量热法研究牛血清白蛋白与十二烷基二羟乙基甲基溴化铵的相互作用
Isothermal titration calorimetric study on interactions between bovine serum albumin and N-dodecyl-di(2-hydroxyehtyl)-methyl ammonium bromide
摘要
Abstract
Interaction enthalpies between bovine serum albumin (BSA) and N-dodecyl-di(2-hydroxyehtyl)-methyl ammonium bromide (DDHAB) have been determined in an isothermal titration calorimeter. The influences of temperature, concentrations of surfactant and the added salt in the solutions on the interaction enthalpy have been discussed. The interaction enthalpies between BSA and a surfactant are resulted from the cooperative effects of electrostatic interaction, dehydration, hydrophobic interaction and surfactant micellization. The interaction enthalpy changes against the surfactant concentration with two endothermic peaks observed in the regions before and after the critical micelle concentration (CMC). The addition of NaCl in the surfactant solution can lead to the value change of the interaction enthalpy and the position change of the endothermic peaks.关键词
等温滴定量热法/牛血清白蛋白/表面活性剂/相互作用Key words
isothermal titration calorimetry (ITC)/ bovine serum albumin/ surfactant/ interaction分类
化学化工引用本文复制引用
胡晓环,童威,邵爽,雷群芳,方文军..等温滴定量热法研究牛血清白蛋白与十二烷基二羟乙基甲基溴化铵的相互作用[J].浙江大学学报（理学版）,2011,38(5):525-529,5.基金项目
国家自然科学基金资助项目(20673098,21073164) （20673098,21073164）
浙江省自然科学基金资助项目(Y4080201). （Y4080201）
