实用妇产科杂志2011，Vol.27Issue(10)：754-758,5.
信号诱导增殖相关蛋白1和16、18型人乳头瘤病毒E6蛋白在宫颈癌中的表达及临床意义
Expression and Clinical-Pathology Significance of HPV16/18 E6 and SIPA1 Protein in Cervical Cancer
李东林 1蔡晶 2况燕 1曹晋 1王泽华3
作者信息
- 1. 华中科技大学同济医学院附属协和医院,湖北武汉430022
- 2. 遵义医学院附属医院,贵州遵义563003
- 3. 广西医科大学第一附属医院,广西南宁530021
- 折叠
摘要
Abstract
Objective;To explore the expression of early protein E6 of human papillomavirus type 16 and 18(HPV16/18E6) and signal induced proliferation associated protein 1 (SIPA1) in cervical cancer, and its relationship to clinic-pathology. Methods;The expression of HPV16/18 E6 and SIPA1 were detected in C33A,HT3,SiHa,HeLa and CaSki cell lines,and paraffin embeded cervical cancer tissues from 174 patients and 40 normal cervical tissues by western blot and immunohistochemistry Envision method respectively. The relationship of HPV16/18 E6 and SIPA1 to pelvic lymph node metastasis and the correlation between expression of SIPA1 and HPV16/18 E6 were analyzed. Results: There was negative correlation between SIPA1 and HPV16/18E6 in cervical cancer cell lines. The positive rate of SIPA1 in normal cervical and cervical cancer tissues was 87. 5% and 58. 6% respectively (x2 = 11. 78, P = 0. 001). In patients with or without lymph node metastasis, the positive rate of SIPA1 was 19. 0% and 71. 2% respectively (x2 = 21. 45,P =0. 000). The risk of lymph node metastasis in patients with negative SIPA1 was higher than positive patients (OR=5. 011, P ＜ 0. 01). The positive rate of HPV16/18 E6 in normal cervix and cervical cancer was 30. 0% and 79. 3% respectively (x2 =37.72,P=0. 000), There was significant difference between the cases with or without lymph node metastasis (x2 =11. 31 ,P = 0. 001). The risk of lymph node metastasis in patients with HPV16/18 E6 positive was higher than negative cases (OR= 14. 794, P ＜ 0. 01). There was negative correlation between SIPA1 and HPV16/18 E6 in cervical cancer (r= -0. 249,P＜0. 001). Conclusions: The down regulation of SIPA1 in cervical cancer is associated with human papillomavirus infection and lymph node metastasis. The efficient HPV16/18 E6 can lead to inhibit SIPA1 expression and SIPA1 can be used as a prognostic marker for lymph node metastasis of cervical cancer.关键词
宫颈癌/肿瘤转移/信号诱导增殖相关蛋白1/16、18型人乳头瘤病毒E6蛋白/免疫组织化学Key words
Cervical cancer/Metastasis/Signal induced proliferation associated protein 1 (SIPA1)/HPV16/18 E6/lmmunohistochemistry分类
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李东林,蔡晶,况燕,曹晋,王泽华..信号诱导增殖相关蛋白1和16、18型人乳头瘤病毒E6蛋白在宫颈癌中的表达及临床意义[J].实用妇产科杂志,2011,27(10):754-758,5.
