中国农业科学2011，Vol.44Issue(18)：3877-3885,9.DOI:10.3864/j.issn.0578-1752.2011.18.019
基于AFLP技术的12个蜜蜂种群遗传多样性分析
Analysis of Genetic Diversity in Twelve Honeybee Populations Based on AFLP Technique
摘要
Abstract
[ Objective ] The objective of this study is to evaluate the population genetic diversity and differentiation among Apis cerarta, Apis dorsata and Apis mellifera in China. [ Method ] DNA gene pools were genotyped by AFLP with nineteen pairs of primer combinations in twelve honeybee populations, which are individually ten Apis cerarta from ten provinces of China, one Apis dorsata and one Apis mellifera.[ Result] The genetic similarity coefficient among the different honeybee species was low. However, the coefficient among the different populations of Apis cerana was high. Compared with A. mellifera, the coefficient Apis cerana is 0.2335-0.2823, but with Apis dorsata, the coefficient is 0.2439-0.2871. Between the A. mellifera and Apis dorsata, the coefficient is 0.2650. Among the different populations of Apis ceranas, the coefficient is 0.3639-0.6134. According to the cluster map, the twelve populations can be distinctly divided into three branches, they are Apis mellifera , Apis dorsata and Apis cerana. However, among the different populations of Apis cerana, the honeybees from Jilin and Jiangxi provinces belong to a sub-branch, those from Hainan, Fujian, Guangdong and Yunnan belong to another sub-branch, those from Gansu, Shanxi, Beijing and Sichuan provinces belong to another sub-branch. [Conclusion) Genetic divergence was apparent among Apis mellifera, Apis dorsata and Apis cerana. The coefficient was higher among the different geographic Apis cerana in China. The Apis cerana in Jilin province was a unique group.关键词
蜜蜂/基因池/AFLP分析/种群遗传多样性Key words
honeybees/ gene pool/ AFLP analysis/ population genetic diversity引用本文复制引用
张春香,张学锋,陈廷珠,滕跃中,刘文忠,姜玉锁..基于AFLP技术的12个蜜蜂种群遗传多样性分析[J].中国农业科学,2011,44(18):3877-3885,9.基金项目
山两省科技攻关项口(20090311038-1) （20090311038-1）
