中国农业科学2011，Vol.44Issue(18)：3886-3894,9.DOI:10.3864/j.issn.0578-1752.2011.18.020
2008-2010年中国华东地区PRRSV ORF5基因遗传变异分析
Genetic Variation of the ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in East China from 2008 to 2010
摘要
Abstract
[ Objective ] A total of 260 swine samples of dead or sick pigs obtained from Jiangsu, Anhui, Shanghai, Shandong, Zhejiang, Fujian and Jiangxi provinces of China during 2008-2010 were detected for PRRSV. And ORF5 genes of some isolates were amplified and sequenced for understanding the molecule epidemiology and the genetic evolution of porcine reproductive and respiratory syndrome virus (PRRSV) in East China. [Method] PRRSV was identified by RT-PCR from samples. Sequences of ORF5 of 36 PRRSV positive samples was amplified and analyzed with other 15 strains available on NCBI GenBank. [Result] A total of 118 samples were positive and the positive rate was 45.4%. Sequence analysis showed that the 36 isolates of this study belong to the North American-type PRRSV strains and were closely related to the highly pathogenic PRRSV (HP-PRRSV) with 94.6-100% amino acid sequence identities. The sequence analysis combined with the phylogenetic analysis indicated that all these North American-type PRRSV strains in East China were further divided into five subgenotypes, subgenotype III showed colser identity with HP-PRRSV; almost all subgenotypes were found to be variable in the primary neutralizing epitope and subgenotypes III and IV had more glycosylation sites than others. Although these 36 isolates were collected from different provinces in East China, there were no obvious relations between the distribution of PRRSV and the region. [Conclusion] The PRRSV infection was widespread and HP-PRRSV was the popular strain in East China during 2008-2010. However some different genetic characteristics appeared in the genomes, the genetic evolution was relatively stable. There exists a cross-cutting phenomenon on the genetic relationship of PRRSV isolates obtained from different provinces. Subgenotype IV and V only appeared in some provinces, but the distribution of PRRSV did not show apparent geographical characteristics.关键词
猪繁殖与呼吸综合征( PRRS)/华东地区/ORF5基因/遗传变异Key words
porcine reproductive and respiratory syndrome (PRRS)/ East China/ ORF5 gene/ genetic variation引用本文复制引用
王小敏,吕立新,郭容利,周俊明,李彬,何孔旺,张文文,陈蔚,茅爱华,俞正玉,温立斌,倪艳秀,张雪寒..2008-2010年中国华东地区PRRSV ORF5基因遗传变异分析[J].中国农业科学,2011,44(18):3886-3894,9.基金项目
江苏省农业科技自主创新重点领域(cx(09)619)、江苏省农业科技自主创新资金(cx(10)424) （cx(09）
