中国农业科学2011，Vol.44Issue(18)：3895-3902,8.DOI:10.3864/j.issn.0578-1752.2011.18.021
氧化应激在镉致大鼠肝细胞凋亡中的作用
The Role of Oxidative Stress on the Apoptosis of Rat Hepatocytes Induced by Cadmium
摘要
Abstract
[Objective] The role of oxidative stress on the apoptosis of rat hepatocytes induced by cadmium was studied. [Method] Rat hepatocytes were isolated by a two-step perfusion technique. After 24 h planting, hepatocytes were treated with cadmium acetate, or treated with Cd in the presence or absence of Z-VAD-fink or NAC. Cell viability was measured with MTT assay, the apoptosis, reactive oxygen species (ROS) generation, mitochondrial membrane potential (△Ψm) collapse were measured by flow cytometry. The hepatocytes hotnogenate was prepared to detect the levels of caspase-3, malondialdehyde (MDA) and reduced glutathione hormone (GSH) in spectro photometric assay. [Result] The results showed that cellular viability decreased and apoptosis rate increased significantly or very significantly (P＜0.05 or P＜0.01) in a dose-dependent manner after exposure to 2.5, 5 and 10 μmol·L-1 Cd. Cd (2.5 and 5 μmol·L-1) induced ROS generation significently in 1.5 hours (P＜0.05 or P＜0.01). Cd induced △Ψm collapse. NAC effectively protected hepatocytes against apoptosis induced by Cd. Cd induced ROS generation and △Ψm collapse were blocked by NAC (P＜0.05). The GSH content decreased with the increase of the content of Cd. There was very significant difference in partial groups than the control group at 12 h (P＜0.01), but GSH content increased with the increase of the dose of Cd at 24 h. The activities of MDA level increased. The caspase-3 activity didn't increase. No effect of Z-VAD-fink on apoptosis was observed. [Conclusion] These results showed that ROS generation and oxidative stress by Cd triggers apoptosis via caspase-independent pathway.关键词
镉/大鼠肝细胞/细胞凋亡/氧化应激Key words
cadmium/ rat hepatocytes/ apoptosis/ oxidative stress引用本文复制引用
汪纪仓,刘学忠,袁燕,裔传卉,卞建春,刘宗平..氧化应激在镉致大鼠肝细胞凋亡中的作用[J].中国农业科学,2011,44(18):3895-3902,8.基金项目
江苏省自然科学基金(BK2008214)、江苏省高校“青蓝工程”中青年学术带头人培养对象资助项目(2006) （BK2008214）
