畜牧兽医学报2011，Vol.42Issue(8)：1057-1062,6.
中国美利奴羊PROP1和PRLR基因的表达分析
The Expression Analysis of PROP1 and PRLR Genes in Chinese Merino Sheep
摘要
Abstract
In order to investigate the distribution profile in different tissues and the development expression patterns of PROP1 and PRLR genes in Chinese Merino sheep. Fluorescent real-time quantitative PCR was applied to investigate the mRNA distribution profile of sheep PROP1 and PRLR genes in thirteen adult Chinese Merino sheep tissues, and to compare the developmental expression patterns of PROP1 gene in pituitary and PRLR gene in pituitary, ovary, testis and skin of Chinese Merino lambs at six growth stages (0, 7, 14, 30, 60 and 90 days). The results showed that the expression of PROPl gene only existed in the pituitary,while the expression of PRLR gene was observed in all of detected tissues and it differed in different tissues, which were significantly higher in uterus and hypothalamus than that in other detected tissues (P<0. 01). In addition, in pituitary gland, significantly higher expression level of PROPl gene was observed at 7 days than that at 30 (P<0. 01), 14 and 60 days (P<0. 05). The expression patterns of PRLR gene, in pituitary gland, was relatively higher at day 30 and then declined rapidly, with no significant difference among the ages (P>0. 05) ; in ovary tissue, the expression patterns of PRLR gene decreased rapidly at 7 days then increased, and at 90 days was significantly higher than that at 0 (P<0. 01) , 14 and 30 days (P<0. 05); in testis tissue, the expression level of PRLR increased gradually with a little fluctuant; in skin tissue, the expression of PRLR showed significantlyhigher level at early ages than that at later stages (P<0. 01). These results indicate that the mRNAs of PR0P1 and PRLR genes exhibits specific tissue expression difference and developmental expression changes; and it may play an important role in sheep reproductive traits development.关键词
中国美利奴羊/PROP1基因/PRLR基因/荧光实时定量PCR/组织表达谱/发育变化Key words
Chinese Merino sheep/ PROP1 gene/ PRLR gene/ fluorescent real-time quantitative RT-PCR/ tissue distribution profile/ development change分类
农业科技引用本文复制引用
曾献存,陈韩英,贾斌,赵宗胜,惠文巧,王尊宝..中国美利奴羊PROP1和PRLR基因的表达分析[J].畜牧兽医学报,2011,42(8):1057-1062,6.基金项目
国际科技合作项目(2007DFB30420) （2007DFB30420）
