色谱2011，Vol.29Issue(8)：750-754,5.DOI:10.3724/SP.J.1123.2011.00750
高效液相色谱法测定猪组织中残留的咪唑苯脲
Determination of imidocarb residue in swine tissues by high performance liquid chromatography
摘要
Abstract
A quantitive method for the determination of imidocarb residue in edible tissues of swine by high performance liquid chromatography (HPLC) was developed. The analyte was digested by j8-glucosidase first, and then extracted with 1 mol/L hydrochloric acid. The aqueous phase was back-extracted with the mixture of hexane-isoamyl alcohol (3:2, v/v) for the purification. The mobile phases were acetonitrile (phase A) and 0. 007 5 mol/L 1 -pentansulfonic acid sodium aqueous solution containing 0. 1% triethylamine, adjusted to pH 3. 0 with glacial acetic acid (phase B). The analyte was detected by ultraviolet absorption spectroscopy after the separation was achieved on a C18 RP column. The linear range was 10 - 10 000 μg/L, and the correlation coefficient was more than 0. 999. The limit of detection (LOD) was 10 μg/kg, and the limit of qualification (LOQ) was 20 μg/kg. The mean recoveries of imidocarb in swine tissues at the added levels of LOQ, MRL (maximum residue limit) and 2MRL ranged from 80.04% to 110. 32%, and the relative standard deviations (RSDs) of intra- and inter-day analyses ranged from 0. 82% to 10. 00%. The method is simple and sensitive for the quantification of imidocarb residue in swine tissues.关键词
高效液相色谱法/咪唑苯脲/兽药残留/猪/可食性组织Key words
high performance liquid chromatography (HPLC)/ imidocarb j veterinary drug residue / swine/ edible tissues分类
化学化工引用本文复制引用
于慧敏,黄显会,钟家林,杨刚,方秋华..高效液相色谱法测定猪组织中残留的咪唑苯脲[J].色谱,2011,29(8):750-754,5.基金项目
2009年农业行业标准制定和修订计划项目 （）
