色谱2011，Vol.29Issue(8)：755-761,7.DOI:10.3724/SP.J.1123.2011.00755
固相萃取-高效液相色谱法同时测定传统禽肉制品中的9种杂环胺类化合物
Simultaneous determination of 9 heterocyclic aromatic amines in poultry products by solid-phase extraction-high performance liquid chromatography
摘要
Abstract
A method was developed for the simultaneous determination of 9 heterocyclic aromatic amines (HAAs) including 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), 2-amino-3,4-dimethylimidazo[ 4,5-f] quinoline ( MeIQ), 2-amino-3 , 8-dimethylimidazo [ 4, 5-f] quinoxaline (MelQx), 2-amino-3, 4, 8-trimethyl-imidazo [4,5-f] quinoxaline (4, 8-DiMeIQx) , 2-amino-l-methyl-6-phenylimidazo[4,5-b)]pyridine (PhIP), 3-amino-l-methyl-5ff-pyrido [4, 3-6 ] indole (Trp-p-2), 3-amino-l,4-dimethyl-5H-pyrido[4,3-6] indole (Trp-p-1), 9H-pyrido[ 3 ,4-6] indole (Norharman), 1-methyl-9H-pyrido[3,4-b] indole (Harman)) in poultry products using solid-phase extraction-high performance liquid chromatography (SPE-HPLC). The performance of 3 different organic extraction solutions, 2 solid phase extraction (SPE) procedures, three different reversed-phase columns and five different mobile phases were tested for optimizing separation conditions of the 9 HAAs from poultry products. In the end, ethyl acetate was selected as the extraction solution, and the extract was purified with propylsulfonic acid silica ( PRS) and C,, SPE columns. The analysis was performed on a TSK-gel ODS-80TM column using a gradientelution with the mobile phases of 0. 05 mol/L aqueous acetic acid-ammonium acetate buffer (pH 3.4) and acetonitrile. The results showed that the average recoveries (n =6) of the 9 HAAs spiked in meat samples at 3 levels ranged from 60. 47% to 90. 55% with the relative standard deviations ( RSDs) between 0.49% and 9. 74%, and the limits of detection ( LODs, S/N = 3) were in the range of 0. 1 -3.6 μg/kg. The method is simple, rapid, accurate and sensitive enough for the analysis of HAAs in poultry products.关键词
固相萃取/高效液相色谱/杂环胺/禽肉制品Key words
solid-phase extraction (SPE)/ high performance liquid chromatography (HPLC)/ heterocyclic aromatic amines (HAAs)/ poultry products分类
化学引用本文复制引用
邵斌,彭增起,杨洪生,吴光红,姚瑶,万可慧..固相萃取-高效液相色谱法同时测定传统禽肉制品中的9种杂环胺类化合物[J].色谱,2011,29(8):755-761,7.基金项目
国家现代农业(肉牛)产业技术体系专项项目(No.nycytx-38)、江苏省科技基础设施计划项目(No.BM2008158)和江苏省水产三项工程项目(No.PJ2010-52) （肉牛）
