中国畜牧兽医2011，Vol.38Issue(8)：63-66,4.
犬瘟热病毒贵州分离株N基因的克隆及序列分析
Cloning and Sequence Analysis of N Gene of Canine Distemper Virus Isolated from Guizhou
摘要
Abstract
Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank.and the N gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) from CDV-GZ1 strain in Guizhou. The amplified fragment was cloned and analyzed. The results showed that the length of N gene was 1572 bp. The homology of coding amino acid between this strain and CDV Shuskiy and 01-2689 strain was 98. 9% and 97. 1% respectively,while above 95% among other domestic strain. It illustrated that N protein have highly conservation property.关键词
犬瘟热病毒/N蛋白/克隆/序列分析Key words
canine distemper virus /N protein/clone/sequence analysis分类
生物科学引用本文复制引用
周莉,刘志杰,曾智勇,汤德元,李谦,王彬,张晓杰,甘振磊,王凤..犬瘟热病毒贵州分离株N基因的克隆及序列分析[J].中国畜牧兽医,2011,38(8):63-66,4.基金项目
贵州大学博士基金(X060054). （X060054）
