军事医学2011，Vol.35Issue(12)：921-927,7.
弗氏志贺菌2a GadB蛋白的功能研究
GadB of Shigella flexneri 2a:a pilot study
摘要
Abstract
Objective To investigate the potential functions of gadB in Shigella flexneri 2a. Methods Gene gadB was knocked out from S.flexneri 2a stain 301 by λ-Red recombinant system. The biochemical phenotypes and the virulence of the mutant strain was tested. Then, two-dimensional gel electrophoresis ( 2-DE ) was used to identify the differentially ex-pressed proteins between the wild-type strain and the mutant strain. Results The null-mutant strain of gadB was success-fully constructed that could not use glycerol as the only carbon source. Sereny test revealed that the virulence of gadB mu-tant was slightly weaker than that of the wild-type strain. A total of 10 proteins were identified as differentially expressed proteins according to the comparative proteomic results. Conclusion Deletion of gadB can influence the expression of some proteins. These data provide some clues to the function research of gadB.关键词
志贺菌,弗氏/GadB蛋白/λ-Red重组系统/基因敲除/双向电泳Key words
Shigella fkxneri/ GadB/ λ-Red recombinant system/ knockout/ two-dimensional gel electrophoresis分类
医药卫生引用本文复制引用
赵天,朱力,冯尔玲,马姗姗,廖祥儒,王恒樑..弗氏志贺菌2a GadB蛋白的功能研究[J].军事医学,2011,35(12):921-927,7.基金项目
国家自然科学基金资助项目(81071324,81171531) （81071324,81171531）
国家973计划(2011CB504901) （2011CB504901）
