军事医学2011，Vol.35Issue(12)：933-936,4.
亲水色谱-质谱联用(HILIC-MS)定量测定大鼠血浆中美金刚浓度及药代动力学研究
Quantification of memantine in rat plasma by hydrophilic interaction chromatography/tandem mass spectrometry and its application in pharmacokinetics
摘要
Abstract
Objective To establish a liquid chromatography/tandem mass spectrometry employing hydrophilic interac-tion chromatography with mass spectrometry( HILIC-MS )method for determining memantine in rat plasma. Methods The sample preparation consisted of a protein precipitation extraction and was determined by HILIC-MS. The positive ion selecte-ol ion monitoring ( SIM ) mode was performed and the detective ions m/z 180 and 285 were used to measure memantine and Ro 64-0802 ( I. S. ). The chromatographic separation was achieved using an Amide-80( 4. 6 mm x 100 mm,5 μm )analytical column with an isocratic mobile phase composed of acetonitrile-water-formic acid( 70: 30: 0. 1, v/v/v, 0. 35 ml/min ). Results The method was linear over a concentration range of 2 to 500 ng/ml. The LLOQ was 2 ng/ml. The average inter-day and intra-day precision values was in 3. 9% and 5. 6% , while the accuracy value was in 84. 9% - 111. 2% . Conclu-sion Using this method, retention time is greatly improved, and the matrix effects are decreased by HILIC-MS. This method has been successfully applied to the preclinical and clinical research of memantine.关键词
亲水色谱-质谱联用/大鼠血浆/美金刚/阿尔茨海默病Key words
hydrophilic interaction chromatography with mass spectrometry/ rat plasma/ memantine/ Alzheimer's disease分类
化学化工引用本文复制引用
李迎,阮金秀,张振清..亲水色谱-质谱联用(HILIC-MS)定量测定大鼠血浆中美金刚浓度及药代动力学研究[J].军事医学,2011,35(12):933-936,4.基金项目
"国家重大新药创制"科技重大专项综合性新药研究开发技术大平台(2009ZX09301-002,2009ZX09304-004) （2009ZX09301-002,2009ZX09304-004）
国家自然科学基金资助项目(81102498) （81102498）
