军事医学2011，Vol.35Issue(12)：909-912,4.
血管平滑肌细胞钙化过程中DNA损伤响应分子p21、p53和c-abl的变化
Change in DNA damage response molecules during arterial calcification
岳翔 1程孝中 2吴青 1黄蓓 2张艳红 3温朝阳 1钟辉2
作者信息
- 1. 安徽大学生命科学学院,合肥,230039
- 2. 军事医学科学院生物工程研究所,北京,100850
- 3. 解放军总医院,北京100039
- 折叠
摘要
Abstract
Objective To investigate the change in DNA damage response molecules during arterial calcification. Methods Expression of p21 ,p53 and c-abl was detected by RT-PCR. The phosphorylation level of p53 protein and chkl protein was detected by Western blot. Results The mRNA level of p21 ,p53 and c-abl was upregulated at the point of 2,4 and 6 days and descended from the eighth day in the vacular smooth cells after treatment with 10 mmol β-glycerol phos-phate. The phosphorylation level of p53 protein and chkl protein was also up-regulated in the first 6 days. Conclusion The mRNA level of p21,p53 and c-abl and the phosphorylation level of p53 protein and chkl protein are up-regulated in the early stage of calcification, suggesting that calcification may be correlated with DNA damage response.关键词
肌,平滑,血管/钙化/DNA/损伤/p21/p53/c-ablKey words
muscle, smooth, vascular/ calcification/ DNA damage/ p21/ p53 / c-abl分类
医药卫生引用本文复制引用
岳翔,程孝中,吴青,黄蓓,张艳红,温朝阳,钟辉..血管平滑肌细胞钙化过程中DNA损伤响应分子p21、p53和c-abl的变化[J].军事医学,2011,35(12):909-912,4.
