农业生物技术学报2011，Vol.19Issue(4)：597-605,9.DOI:10.3969/j.issn.1674-7968.2011.04.002
供体细胞和曲古抑菌素(TSA)对奶牛克隆胚胎发育的影响
Effect of Donor Cell and Trichostatin A (TSA) on Development of Cloned Dairy Cattle Embryos
摘要
Abstract
The objective of present study was to investigate the effects of treatments to donor cells with fresh digestion (FD), cryopreservation/thawning (CT), trichostatin A (TSA) and durations of culture using TSA-Crlaa medium on in vitro development of dairy cow cloned embryos. In addition, some somatic cell cloned embryos were transferred to surrogates in heat to evaluate the in vivo developmental competence. The results showed that pretreatment of donor cells using TSA could significantly increase both cleavage and blastocyst rates of embyos (P<0.05) compared with FD and CT group, while no significant difference was found between FD and CT group. When cloned embryos were subjected to TSA treatment in Crlaa for different times (0, 24 , 48 and 60 h), the results showed that the blastocyst rate in the 60 h group was the highest (36.11 ± 1.78%) compared with the other groups (P<0.05). While the reconstructed embryos derived from donor cells treated with TSA for 24 h were continually cultured in TSA for different times (24, 48 and 60 h), the results showed that the blastocyst rate(37.39±1.78%) in the 60 h group was significantly higher than that of 24 h (25.48±1.34%) group (P<0.05). Finally, when the cloned embryos from different groups were respectively transferred to 40 natural estrus recipients, no significant difference in terms of pregnancy rate among groups was found, however, a viable cloned calf was successfully obtained from TSA-treated donor cells and cloned embryo. Therefore, cloned embryos treated with optimized methods can development to term.关键词
曲古抑菌素(TSA)/体细胞核移植/奶牛Key words
Trichostatin A/Somatic cell nuclear transfer/Dairy cow引用本文复制引用
章美玲,张运海,陶勇,刘亚,曹鸿国,李运生,Oto Hanus,Josef Kucera,章孝荣..供体细胞和曲古抑菌素(TSA)对奶牛克隆胚胎发育的影响[J].农业生物技术学报,2011,19(4):597-605,9.基金项目
本研究由国家高技术研究发展计划(863)重点项目(No.2008AA 101003)、安徽省十一五重大科技专项(No.08010301066)、安徽省国际科技合作计划项目(No.08080703034)和安徽省科技攻关项目(No.08010301066)共同资助 （863）
