农业生物技术学报2011，Vol.19Issue(4)：649-653,5.DOI:10.3969/j.issn.1674-7968.2011.04.008
黄瓜果实苦味(Bt)基因的插入缺失(Indel)标记
The Insertion-deletion(Indel) Marker Linked to the Fruit Bitterness Gene(Bt) in Cucumber
摘要
Abstract
To identify Insertion-deletion (Indel) markers tightly linked to the fruit bitterness (Bt) gene in cucumber (Cucumis sativus L.), a F2 population including 184 individuals derived from two cucumber inbred lines 931 (btbt) and 46GBt (BtBt) was used in this study. Results showed that the inheritance of fruit bitterness in cucumber was controlled by one dominant gene Bt. Based on primary mapping of Bt gene and re-sequence of two parental lines, Bioinformatics was employed to analyze the sequence difference between the two parental lines in the primary mapping region of Bt gene. Then seven Indel markers were developed. The InDel marker Bt-InDel-I tightly linked to Bt gene with 0.8 cM was identified after analysis on F2 population with 184 individuals using the seven Indel markers. The veracity of Bt-InDel-1 was tested using another F2 population with 58 individuals, and the accuracy rate for this marker was 94.8%. The results indicated that the Bt-InDel-1 marker can be used in non-bitter MAS cucumber breeding and fine-mapping of Bt gene.关键词
黄瓜/果实苦味Bt基因/Indel标记Key words
Cucumber/Fruit bitterness (Bt) gene/Indel marker引用本文复制引用
张圣平,苗晗,程周超,张忠华,武剑,孙日飞,顾兴芳..黄瓜果实苦味(Bt)基因的插入缺失(Indel)标记[J].农业生物技术学报,2011,19(4):649-653,5.基金项目
本研究由国家自然科学基金(No.30900989)、国家现代农业产业技术体系项目(No.Nycytx-35)、农业部“948”研究项目(No.2008-Z42)、中央级科研院所基本科研业务费专项(No.201011)和农业部园艺作物遗传改良重点开放实验室共同资助 （No.30900989）
