农业生物技术学报2011，Vol.19Issue(4)：763-770,8.DOI:10.3969/j.issn.1674-7968.2011.04.024
鸭早期胚胎发育相关基因消减文库的构建与分析
Construction and Analysis of a Subtractive cDNA Library of Duck Early Embryonic Development Related Genes
摘要
Abstract
Animal embryonic development is regulated by many genes. In order to explore the molecular mechanism of duck early embryonic development, we constructed the subtractive library of duck early embryonic development related genes to screen the related candidate genes of duck early embryonic development. Two different periods (6 and 20 h) duck (Anas platyrhynchos) embryo were used respectively as the driver and tester, and a designated duplex-specific nuclease (DSN) mediated subtractive hybridization method was employed to construct the subtractive library of duck early embryonic development related genes, and the genes were sequenced and analyzed by bioinformatics. Results showed that 54 genes were obtained by DNA sequencing from 216 positive clones picked randomly. Their average length was 1 084 bp. Nucleotide BLAST homological analysis showed that 44 genes had similarities to known genes. Gene ontology showed that these genes were related with a variety of biological pathways, such as metabolism, establishment of cellular localization, biological regulation> etc. And these genes played very important roles during early embryonic development of duck in many different aspects (such as nervous system development, immune regulation, transcriptional regulation, etc.). We conclude that isolation and identification of these differentially expressed genes will contribute to the future related rese archof poultry early embryonic development.关键词
鸭/早期胚胎发育/双链特异性核酸酶(DSN)/消减杂交Key words
Duck/Early embryonic development/Designated duplex-specific nuclease(DSN)/Subtractive hybridization引用本文复制引用
钟灵秀,刘雅丽,李进军,沈军达,卢立志,石放雄..鸭早期胚胎发育相关基因消减文库的构建与分析[J].农业生物技术学报,2011,19(4):763-770,8.基金项目
本研究由农业部现代农业产业技术体系建设专项资金(No.NYCYTX-45-02)和浙江省重大科技攻关项目(No.2005C12005-01,No.2008C14073)共同资助 （No.NYCYTX-45-02）
