中南大学学报（自然科学版）2011，Vol.42Issue(8)：2266-2273,8.
载TGF-β1明胶微球涂层多孔钛对MG63细胞功能的影响
Effects of TGF-β1 gelatin microspheres coated on porous titanium on function of MG63 cells
摘要
Abstract
Gelatin microspheres were made by improved emulsified cold condensation method. Effects of TGF-β1 gelatin microspheres on the proliferation and differentiation of MG63 cells were investigated in vitro, as well as the optimal TGF-β1 concentration. Porous titanium with a porosity of 60% and an inter-connected structure was prepared using a powder injection molding method. A coating of TGF-β1 loaded gelatin microspheres was applied in the pores of the porous titanium surface. MTT assay method was used to evaluate the cytotoxicity of the coating to L929 cells. The pharmacokinetics of the coating was tested with TGF-β1 ELISA kit. In vitro, the effect of the coating on adhesion, proliferation and differentiation of MG63 cells was investigated. The results show that the TGF-β1 concentration affects the function of MG63 cells. In vitro, the proliferation and differentiation of MG63 cells show a positive dose-effect relationship at TGF-β1 concentrations of 0.025-2.500 mg/g. TGF-β1, concentration of 2.500 mg/g is in favor of the differentiation, while the concentration of 25 mg/g inhibites cells' proliferation. Porous titanium samples coated with TGF-β1 gelatin microspheres are nontoxic to L929 cells and are able to maintain a slow-release state during 12 d. The porous titanium implants coated with TGF-β1 loaded gelatin microspheres benefits MG63 cells adhesion, proliferation and differentiation on the 3th, 7th, 14th slot.关键词
多孔钛/载TGF-β1微球涂层/成骨细胞/增殖/分化Key words
porous titanium/ TGF-β1 loaded gelatin microspheres coating/ MG63 cell/ proliferation/ differentiation分类
医药卫生引用本文复制引用
陈良建,汪瑞芳,郭小平,李益民,何浩,李挺..载TGF-β1明胶微球涂层多孔钛对MG63细胞功能的影响[J].中南大学学报（自然科学版）,2011,42(8):2266-2273,8.基金项目
国家自然科学基金资助项目(357705760) （357705760）
国家“863”计划新材料领域专项(2007AA03Z114) （2007AA03Z114）
中南大学粉末冶金国家重点实验创新基金资助项目(2010) （2010）
