中国畜牧兽医2011，Vol.38Issue(8)：71-77,7.
犬瘟热病毒分离株N、H、F蛋白基因的变异分析
Genetic Diversity Analysis of the N, H, F Protein Genes of Canine Distemper Virus Isolate
摘要
Abstract
The nucleoprotein (N), haemgagglutinin protein (H)and fusion protein (F) genes of canine distemper virus (CDV) isolate GS0812-4were obtained by RT-PCR and sequenced after being cloned directly into the pGEM-T Easy. The sequences of N,H and F genes of GS0812-4 were compared with sequences and antigen epitope of other CDV strains in Gen-Bank by BLAST and DNAStar software. The results showed that the N,H and F of GS0812-4 shared 93. 2% to 97. 8% ,90. 3% to 97. 3%,93. 2% to 97.8% of nucleotide homology, and 96. 6% to 98. 7%,91. 3% to 97.9% and 96.6% to 98.7% of amino acid identity with that of referenced strains, respectively. However, The N, H and F genes of the GS0812-4 shared only 93.1% to 93. 8%,89. 4% to 90. 5% and 93.1% to 93. 8% of nucleotide homology, and 96.4% to 97. 3% ,89. 3% to 91.3% and 96. 4% to 97. 3% of amino acid homology with that of vaccine strains, respectively. Phylogenetic tree showed that GS0812-4 was different from the vaccine strain of the branch, it had a recent genetic relationship and MKY-KM08. But GS0812-4 compared with other strains of different genetic distance relationships. H, F protein epitope prediction results showed that GS0812-4 epitope was different from and MKY-KM08 and vaccine strains. Results showed that GS0812-4 was the wild strain, and the GS0812-4 strain was MKY-KM08 belonged to the same genotype, derived from the same strain, but there were still differences between epitopes.关键词
犬瘟热病毒/H蛋白/H蛋白/F蛋白/变异分析Key words
canine distemper virus(CDV) /nucleoprotein(N) /fusion protein(F) /haemgagglutinin protein(H)/genetic diversity分类
生物科学引用本文复制引用
王旭荣,王小辉,张世栋,李世宏,潘虎,严作廷..犬瘟热病毒分离株N、H、F蛋白基因的变异分析[J].中国畜牧兽医,2011,38(8):71-77,7.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(BRF080302). （BRF080302）
