畜牧兽医学报2011，Vol.42Issue(8)：1168-1174,7.
钙离子超载在镉致大鼠肝细胞凋亡中的作用
Cadmium Induced Apoptosis in Rat Hepatocytes by Calcium Overloading
摘要
Abstract
The aims of this study were to explore the effect of [Ca2+]I on the apoptosis of rat hep-atocytes induced by cadmium. Rat hepatocytes were isolated by a two-step perfusion technique. After 24 h planting, hepatocytes were treated with cadmium in the presence or absence of Bapta-AM. Cell viability was evaluated with a colorimetric using MTT level. Cell morphology and apoptosis were photographed under inverted and fluorescence microscope. [Ca2+ ]I, ROS generation and △ψm collapse were evaluated with flow cytometry. The results showed sharp deformation, necrosis and apoptosis in hepatocytes with the dose of Cd increased and time prolonged. Cd induced [Ca2+]I increased, △ψm collapse and ROS generation (P<0. 01). Bapta-AM effectively inhibited Cd-induced cytotoxicity (P<0. 05 or P<0. 01). It was showed that cadmium induced apoptosis in rat hepatocytes by calcium overloading.关键词
镉/大鼠/肝细胞/Ca2+/细胞凋亡Key words
cadmium/ rat/ hepatocytes/ Ca2+/ apoptosis分类
农业科技引用本文复制引用
汪纪仓,孙娅,宋瑞龙,卞建春,刘宗平..钙离子超载在镉致大鼠肝细胞凋亡中的作用[J].畜牧兽医学报,2011,42(8):1168-1174,7.基金项目
江苏省高校“青蓝工程”中青年学术带头人培养对象资助项目(2006) （2006）
江苏省自然科学基金资助项目(BK2008214) （BK2008214）
河南科技大学博士科研究启动基金资助(09001489) （09001489）
