畜牧兽医学报2011，Vol.42Issue(8)：1081-1087,7.
牛乳腺脂肪合成关键酶基因在乳汁体细胞中的表达研究
The Expression of the Related Fatty Acid Synthesis Key Enzyme Genes in Bovine Somatic Cell
摘要
Abstract
To clarify the molecular mechanism of milk fat synthesis, optimizing ruminant raw milk, particularly providing theory basis for nutrition regulation and heredity improving for milk fat. Somatic cells in colostrum milk, mature milk and involution milk were selected and housekeeping gene GAPDH was selected as reference, the semi-quantitive RT-PCR was used to analyze the expression of LPL, CD36, VLDLR, ACSS2, ACSLl, FABP3, ACC, FASN, SCD, AD-FP, XDH and BTN1A1 mRNA in milk. The results showed that the genes LPL, CD36, VLDLR, ACSS2, ACSLl, FABP3, ACC, FASN, SCD, ADFP, XDH and BTNV1A1 mRNA expressed in the colostrum milk, mature milk and involution milk. However the genes ACC and FASN were not detected in mature milk and involution milk. The relative quantitive results showed that the expression level of LPL, CD36, VLDLR, ACSS2, ACSLl, FABP3, ACC, FASN, SCD, ADFP, XDH and BTN1A1 mRNA in mature milk and involution milk were significantly decreased(P<0. 05) , compared with colostrum milk. The transcription level difference of these genes was not significant(P>0. 05) between mature milk and involution milk. These re-suits suggest that the ability of milk fat synthesis in colostrum is higher than that in mature milk and involution milk, and the expression of fatty acid synthetic key genes is related to fat transpor-tant and metabolism of cell.关键词
乳汁/体细胞/脂肪合成关键酶基因/RT-PCRKey words
milk/ somatic cell/ fatty acid synthetic key enzyme genes/ RT-PCR分类
农业科技引用本文复制引用
谢佳喜,朱河水,杨国宇,李宏基,郭豫杰,汪新建,王月影..牛乳腺脂肪合成关键酶基因在乳汁体细胞中的表达研究[J].畜牧兽医学报,2011,42(8):1081-1087,7.基金项目
“十一五”国家科技攻关计划子课题(2006BAD04A03-10) （2006BAD04A03-10）
河南省科技攻关课题(072102140002) （072102140002）
