畜牧兽医学报2011，Vol.42Issue(8)：1088-1094,7.
鸡A-FABP基因不同基因型遗传效应及初步验证
Genetic Effect and Preliminary Verification of Different Genotypes in Chicken A-FABP Gene
摘要
Abstract
This experiment was designed to analyze and verify the genetic effect of different genotypes in adipocyte fatty acid-binding protein(A-FABP) gene, A-FABP was one of the important candidate genes for intramuscle fat trait (IMF) in chicken, which provided the base for explorat-ing and utilizating of molecular markers related to this trait. PCR-SSCP technology was used to detect the single nucleotide polymorphisms (SNPs) of A-FABP, and association between its SNPs and IMF content of breast muscle was conducted; then fluorescence quantitative real-time PCR was performed to explore the temporal and spatial expression pattern of different genotypes in progeny populations. A SNP (g. 1763 A>G) in exon3 of A-FABP gene was found, which resulted in the substitution of amino acid (Ser to Asn). In three genotypes of A-FABP gene, IMF contents of individuals with the AA and AB genotypes were significantly higher than that of individuals with the BB genotype (P<0. 05), and the AA genotype was the dominant. No significantly different expression was found between male and female. The expression level in differenttissues was abdominal fat, breast muscle, heart and liver according to the descending order. At different periods, the A-FABP expression had the same trend among muscle, heart and liver, which reached top point at 4 weeks and decreased from 6 weeks. However, A-FABP expressed in abdominal fat reached top point at 10 weeks and decreased from 12 weeks. In addition, the expression level had significant differences in the same tissue among the different A-FABP genotypes. The expression level of individuals with BB genotype was the lowest in all tissues, which was consistent with the results of association analysis between the detected SNPs and IMF content of breast muscle. Thus, the BB genotype had close association with the low IMF content of breast muscle in chicken. The results indicate that the mutation of g. 1763 A>G in exon 3 of A-FABP could be used as an available candidate marker affecting IMF content trait in chicken.关键词
鸡/A-FABP/基因型/遗传效应/验证Key words
chicken/ A-FABP/ genotype/ genetic effect/ verification分类
农业科技引用本文复制引用
常国斌,周琼,栾德琴,陈蓉,王克华,窦套存,胡玉萍,陈国宏..鸡A-FABP基因不同基因型遗传效应及初步验证[J].畜牧兽医学报,2011,42(8):1088-1094,7.基金项目
国家自然科学基金(30972088) （30972088）
江苏省自然科学基金(BK2009190) （BK2009190）
江苏省农业科技支撑计划(BE2008359) （BE2008359）
