畜牧兽医学报2011，Vol.42Issue(8)：1101-1106,6.
鸭胚卵内注射rhIGF-1对其出壳后卵泡抑素基因表达及胸肌发育的影响
The Effect of in ovo Injecting rhlGF-1 into Egg on the Expression of Follistatin Gene after out of Shell and the Muscle Development in Duck
摘要
Abstract
In order to investigate the effect of different IGF-1 concentrations on the expression of Follistatin(FST), the different concentration rhIGF-1 with 120, 100, 80 and 0 ng · mL-1 (the control group) were injected into the duck egg using in ovo injecting method, and then the expression of FST was detected using QPCR technique. The results showed that the body weight of ducklings at the age of two days among different treatment groups displayed no significant difference (P>0. 05); while the breast muscle weight and breast muscle rate increased with the concentration of rhIGF-1 raising, and it displayed a significant difference between the 120 ng · mL-1 and the control groups (P<0. 05). The diameter and cross section area of muscle fibers in 120 ng · mL-1 rhIGF-1 treatment were the most maximum with a significant difference comparing to control (P<0. 05). The expression level of the FST in duckling breast muscle increased as the concentration of rhIGF-1 was raised, and the expression level of FST reached its highest in 120 ng · mL-1 rhIGF-1 treatment group with a significant difference comparing with other groups (P<0. 05). After analyzing, the expression of FST is induced by IGF-1 in a dose-dependentmanner, and the higher concentration of IGF-1 also promote duck breast muscle development.关键词
雏鸭/卵内注射/rhIGF-1/肌纤维/FollistatinKey words
duckling/ inovo injecting/ rhIGF-1/ myofiber/ Follistatin分类
农业科技引用本文复制引用
丁庆峰,刘贺贺,陈禧,张荣萍,万一,李林森,李亮,王继文..鸭胚卵内注射rhIGF-1对其出壳后卵泡抑素基因表达及胸肌发育的影响[J].畜牧兽医学报,2011,42(8):1101-1106,6.基金项目
国家科技支撑计划项目(2008BADB2B08) （2008BADB2B08）
国家大学生创新性实验项目(081062610) （081062610）
