畜牧兽医学报2011，Vol.42Issue(8)：1193-1200,8.
鹅β-防御素基因克隆与生物学特性的初步分析
Cloning and Initial Characterization of Goose Avian β-defensin
摘要
Abstract
In the present study, avian β-defensin (AvBD) gene was isolated from goose bone marrow tissues by RT-PCR. The complete nucleotide sequence of the gene contained a 201 bp cDNA encoding 66 amino acids. Phylogenetic relationships were analyzed. It was demonstrated that the goose AvBD shared the highest amino acid homology with chicken AvBD5 (84. 8%), and it was named as goose AvBD5. The cDNA of goose AvBD5 was sub-cloned into EcoR I and Sal I digestion sites of pGEX-6p-l vector to construct recombinant plasmid pGEX- AvBD5. The recombi-nant plasmid was transformed into E. coli BL21 and the bacterial was induced with IPTG. It was demonstrated by SDS-PAGE that a 32 ku protein was highly expressed as insoluble bodies in E. coli BL21. Colony counting assays were performed to investigate the antimicrobial activity of recombinant goose AvBD5. The result indicate that the recombinant goose AvBD5 exhibit extensive antimicrobial activity against all of bacterial strains investigated including Gram-positive and Gram-negative organisms. In addition, the results revealed that the recombinant protein retained antimicrobial activity against Pasteurella multocida under different temperatures and pH.关键词
鹅β-防御素5/重组蛋白/抗菌活性Key words
goose β-defensin 5/ recombinant protein/ antimicrobial activity分类
农业科技引用本文复制引用
周财源,蔺利娟,韩宗玺,邵昱昊,刘胜旺,马得莹..鹅β-防御素基因克隆与生物学特性的初步分析[J].畜牧兽医学报,2011,42(8):1193-1200,8.基金项目
国家自然科学基金(30972110) （30972110）
黑龙江省教育厅科研重点项目(11531z05) （11531z05）
