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猪链球菌2型生物被膜的生物学特性研究

孟祥朋 孙建和

畜牧与兽医2011,Vol.43Issue(9):1-7,7.
畜牧与兽医2011,Vol.43Issue(9):1-7,7.

猪链球菌2型生物被膜的生物学特性研究

Characterization of biofilm formation by Streptococcus suis type 2

孟祥朋 1孙建和1

作者信息

  • 1. 上海交通大学农业与生物学院上海市兽医生物技术重点实验室,上海200240
  • 折叠

摘要

Abstract

In this work, biofilm quantification of Streptococcus suis type 2 (SS2) were evaluated by 96-well microtiter plate assays. The results showed that the significant biofilms were formed completely after 60 hours incubation by 3 out of 15 SS2 strains, and the capacity of biofilm formation was influenced by the concentration of glucose in the medium. Exopolysaccharide content in the biofilm was higher than those produced by the planktonic cells. The resistance of biofilm-grown bacteria to ampicillin or amoxicillin was 2500 times more than that of planktonic bacteria. Scanning electron microscopy revealed a dense biofilm structure formed by S. Suis. The investigation about the formation of 5. Suis biofilm will provide the basis for further research on pathogenic mechanism and removal of biofilm.

关键词

猪链球菌/生物被膜/胞外多糖/耐药性

Key words

Streptococcus suis/ biofilm/ exopolysaccharide/ antibiotic resistance

分类

农业科技

引用本文复制引用

孟祥朋,孙建和..猪链球菌2型生物被膜的生物学特性研究[J].畜牧与兽医,2011,43(9):1-7,7.

基金项目

公益性行业(农业)科研专项(200803016) (农业)

家畜疫病病原生物学国家重点实验室”开放基金课题资助(SKLVEB2010KFKT004). (SKLVEB2010KFKT004)

畜牧与兽医

OA北大核心CSTPCD

0529-5130

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