畜牧与兽医2011，Vol.43Issue(9)：17-20,4.
利用DNA池和测序技术快速筛查SNPs及估算基因频率
Rapidly screening SNPs and estimating allelic frequencies by DNA pooling and sequencing
摘要
Abstract
Three breeds of male pigs ( Coke pig, Yorkshire and White pig) with different varietal characteristics were applied to screen potential single nucleotide polymorphisms (SNPs) related to reproduction trait in 3'UTR, CDS, 5'UTR of ODFP gene based on DNA pooling and direct sequencing of PCR products. Two SNPs (C675T, C786T) were found successfully in the fragment of 994 bp at the region of the CDS. The C675T leaded isoleucine into aspartic acid. Moreover, a simple and rapid method to estimate allelic frequencies of SNPs in each breed by calculating the ratio of allele peak heights was introduced in the present project.关键词
DNA池/测序/SNPs/基因频率Key words
DNA pooling/ sequencing/ SNPs/ allelic frequency分类
农业科技引用本文复制引用
李敬瑞,丁远华,张玉龙,王邦荣,刘若余..利用DNA池和测序技术快速筛查SNPs及估算基因频率[J].畜牧与兽医,2011,43(9):17-20,4.基金项目
贵州大学研究生创新基金资助[校研农(2011)023],贵州省农业科技攻关项目[黔科合NY字(2009) 3073]. （2011）
