麻疯树全植株全长均一化cDNA文库构建及EST序列分析OA北大核心CSCDCSTPCD
Establishment of a normalized full-length cDNA library and analysis of some expressed sequence tags sequencing of Jatropha curcas L.
以麻疯树根、茎、叶、花和果实为材料,使用DSN (duplex-specific nuclease)均一化技术与SMART(switching mechanism at 5' end of RNA transcript)建库技术相结合,构建了麻疯树全植株均一化全长cDNA文库.经检测库容为1.4×106个克隆,插入片段平均长度大于1 kb.随机挑取200个克隆,利用PCR方法测得文库重组率达95％,48.95％的EST(Expressed Seq…查看全部>>
A normalized full-length cDNA library was established using the root,stem,leaf,flower and fruit of Jatropha curcas L. By DSN (duplex-specific uclease)-normalization method combined with SMART (switching mechanism at 5' end of RNA transcript) technique. This library reaches 1. 4 ×106 in capacity; PCR results showed that the average size of inserts was larger than 1000 bp with a recombination rate of 95%. From sequenced 200 clones at random. 48. 95% showed no …查看全部>>
高继海;朱勋路;徐莺;陈放
四川大学生命科学学院,成都610064四川大学生命科学学院,成都610064四川大学生命科学学院,成都610064四川大学生命科学学院,成都610064
生物学
麻疯树均一化全长cDNA文库表达序列标签库容
Jatropha curcas L. normalized full-length cDNA libraryExpressed sequence taglibrary capacity
《四川大学学报（自然科学版）》 2011 (4)
麻疯树核糖体失活蛋白curcin资源研究
935-942,8
“十二五”科技支撑项目(2011BAD22808)自然科学基金(31070301)
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