四川大学学报（自然科学版）2011，Vol.48Issue(4)：935-942,8.DOI:10.3969/j.issn.0490-6756.2011.04.039
麻疯树全植株全长均一化cDNA文库构建及EST序列分析
Establishment of a normalized full-length cDNA library and analysis of some expressed sequence tags sequencing of Jatropha curcas L.
摘要
Abstract
A normalized full-length cDNA library was established using the root,stem,leaf,flower and fruit of Jatropha curcas L. By DSN (duplex-specific uclease)-normalization method combined with SMART (switching mechanism at 5' end of RNA transcript) technique. This library reaches 1. 4 ×106 in capacity; PCR results showed that the average size of inserts was larger than 1000 bp with a recombination rate of 95%. From sequenced 200 clones at random. 48. 95% showed no similarity to known genes,suggesting a potential for further novel gene discovery. Blasted in GeneBank Nr database,97 ES-Ts had high homology to known genes,which participate in basic metabolism and the transportation,cell structure,energy conversion,coenzyme metabolism and transportation,signal transduction. Translation and folding process as well as modification after translation. This is the first time that a normalized full-length cDNA library was established using the whole plant of Jatropha curcas L. As materials.关键词
麻疯树/均一化全长cDNA文库/表达序列标签/库容Key words
Jatropha curcas L. /normalized full-length cDNA library/Expressed sequence tag/library capacity分类
生物学引用本文复制引用
高继海,朱勋路,徐莺,陈放..麻疯树全植株全长均一化cDNA文库构建及EST序列分析[J].四川大学学报（自然科学版）,2011,48(4):935-942,8.基金项目
“十二五”科技支撑项目(2011BAD22808) （2011BAD22808）
自然科学基金(31070301) （31070301）
