四川大学学报（自然科学版）2011，Vol.48Issue(4)：943-948,6.DOI:10.3969/j.issn.0490-6756.2011.04.040
piggyBac共转染系统的构建以及转座活性的验证
Constructing of a piggyBac binary cotransfection system and verifying the activity
摘要
Abstract
The PB inverted terminal repeats (ITR) and PB transposase (Pbase) were cloned from the plasmid PB1156 and atub-pBac-K10. The PB binary cotransfection system consisted of a donor and a helper plasmid. The donor plasmid contained the two ITRs in which Pbase was replaced by a red fluorescent protein (RFP) marker. The helper plasmid carried the Pbase fragment but lacked the ITRs. The result of cotransfection the donor and helper in HEK293 cells indicated that the PB binary cotransfection system is an efficient transposition system. And inverse PCR (IPCR) was performed to recover sequence adjacent to the PB ITR.关键词
转座子/piggyBac(PB)/共转染/IPCRKey words
Transposon/piggyBac(PB)/Cotransfection/IPCR分类
生物科学引用本文复制引用
缪辉,刘华华,吴传芳..piggyBac共转染系统的构建以及转座活性的验证[J].四川大学学报（自然科学版）,2011,48(4):943-948,6.基金项目
国家自然科学基金重大研究计划(90919005) （90919005）
教育部博士点基金(20090181120076) （20090181120076）
