中国烟草科学2011，Vol.32Issue(4)：36-40,5.DOI:10.3969/j.issn.1007-5119.2011.04.009
干旱胁迫对转BnDREB1-5烟草碳氮代谢和渗透调节物质的影响
Effects of Drought Stress on Carbon and Nitrogen Metabolism and Osmotic Adjustment Substances of Transgenic Tobacco with BnDREB1-5 Gene
摘要
Abstract
After drought stress the changes of the carbon and nitrogen metabolism and osmotic adjustment on tobacco with 35S: BnDREBl-5 transformation were analyzed. The results indicated that after physical drought-stress the carbon metabolisms of transgenic tobacco, which included the amylase activity, the invertase activity and the soluble sugar content, increased significantly, and the amylase activity and the soluble sugar content of wild-type increased slightly, but the invertase activity of wild-type decreased. The nitrogen metabolisms of transgenic tobacco, which included the nitrate reductase activity and the soluble protein content, decreased slightly, while those of wild-type tobacco decreased significantly. The BADH activity of transgenic tobacco leaves was detectable and increased after drought stress, but that of wild-type was not detectable. After drought stress, the proline content of transgenic tobacco leaves was 1.08 times as large as that of wild-type. After drought stress the carbon and nitrogen metabolism and defense activity on tobacco with 35S: BnDREBl-5 transformation were obviously better than wild-type.关键词
烟草:DREB转录因子/干旱胁迫/碳氮代谢/渗透调节Key words
tobacco/DREB transcription factor/drought stress/carbon and nitrogen metabolism/osmotic adjustment分类
农业科学引用本文复制引用
胡亚杰,宋魁,王闯,刘卫群,韦建玉..干旱胁迫对转BnDREB1-5烟草碳氮代谢和渗透调节物质的影响[J].中国烟草科学,2011,32(4):36-40,5.基金项目
河南省攻关项目(0624050012) （0624050012）
