应用化学2011，Vol.28Issue(9)：1017-1021,5.DOI:10.3724/SP.J.1095.2011.00489
季铵化N,O-(2-羧乙基)壳聚糖的制备及其抑菌性能
Preparation of Quaternized N, O-(2-Carboxyethyl)Chitosans and Their Antibacterial Activity
摘要
Abstract
A series of amphoteric polymeric electrolyte was prepared and their antibacterial activities and dissolving behaviour in water were investigated. A series of quaternized N, 0-2-carboxyethyl chitosans (QCECs) , which exhibit typical behaviour as amphoteric electrolyte, were obtained through the chemical modifications of N, O-( 2-carboxyethyl ) chitosan ( N, 0-2-CEC ) by 2, 3-epoxypropyl trialkyl ammonium chlorides including glycidyl trimethyl ammonium chloride ( GTMA) , glycidyl triethyl ammonium chloride ( GTEA ) , glycidyl tripropyl ammonium chloride ( GTPA ) , glycidyl tributyl ammonium chloride ( GTBA ) and glycidyl dimethylbenzyl ammonium chloride(GDMBA). Substitution degree of quaternization( DQ) of QCECs was determined by the potentiometry, in which the variations of potential of solution with the volume of AgNO3 aqueous solution were measured. The isoeletric points( pHiep) of ?V,0-2-CEC and its quaternized derivatives were determined by spectrometry method. The antibacterial activities of chitosan derivatives including N,0-2-CEC and QCECs were evaluated according to their minimum inhibition concentrations (MICs) against E. Coli, a typical Gram-negative bacterium, and S. Aureus, a typical Gram-positive bacterium, determined by agar plate method. DQ values of QCECs were estimated in the range of 51. 3% -59. 1% , the pHiep of JV,O-2-CEC and QCECs were in the range of 6. 44 ~ 7. 30 and the introduction of quaternizing, group resulted in the accretion of pHiep. Except the product obtained from the modification of N,0-2-CEC by GTMA, the pHiep values of all other QCECs increased along with their DQ contents. All N, 0-2-CEC and their quaternized derivatives showed improved antibacterial activity against E. Coli and S. Aureus. The MIC values of QCECs were lower than those of N, O-2-CEC. The increase of carbon chain length of alkyl and the presence of dimethyl benzyl in the quaternary ammonium groups could endow QCECs with much better antimicrobial activity.关键词
N,O-(羧乙基)壳聚糖/季铵化改性/等电点/最小抑菌浓度Key words
N, 0-{ carboxyethyl ) chitosan/ quaternizing modification/ isoeletric point/ minimum inhibition concentration分类
化学化工引用本文复制引用
蔡照胜,孙岳明,杨春生,朱雪梅..季铵化N,O-(2-羧乙基)壳聚糖的制备及其抑菌性能[J].应用化学,2011,28(9):1017-1021,5.基金项目
国家973基础研究项目资助(2007 CB936300) （2007 CB936300）
江苏省自然科学基金项目资助(BK2009293) （BK2009293）
