作物学报2011，Vol.37Issue(9)：1676-1682,7.DOI:10.3724/SP.J.1006.2011.01676
花生种子脂肪酶活力定量测定及其与储藏特性的相关性
Quantitative Determination of Peanut Seed Lipase Activity and Its Correlation with Storage Characteristics
摘要
Abstract
Based on colorimetric determination method reported for rice lipase, we established a new method improving and optimizing the measurement for peanut seed lipase activity, with the optimum sampling amount of S mg and substrate concentration of 30 mg ml/-1. The method was validated to be accurate, specific and well repeatable. The lipase activities of 216 peanut germ-plasm resources were detected, with significant difference (P＜0.001) among them. Five with higher lipase activity and five with lower lipase activity were screened, and each two of which were selected to perform an accelerated-aging experiment under high temperature and high moisture. The results indicated that, with extension of storage time, the germination rate of peanut with lower lipase activity decreased slowly, while that of peanut with higher lipase decreased rapidly, showing a negative correlation between seed vigor and peanut lipase activity (correlation coefficient was -0.8875). The study provides a reference for the further research in the effect of lipase on peanut storage characteristics.关键词
花生/脂肪酶活力/定量测定/储藏特性Key words
Peanut/ Lipase activity/ Quantitative determination/ Storage characteristics引用本文复制引用
高奇,蒋岚,张瑛,刘泽,张正竹,吴跃进,禹山林,张琛,滕斌,吴敬德..花生种子脂肪酶活力定量测定及其与储藏特性的相关性[J].作物学报,2011,37(9):1676-1682,7.基金项目
本研究由安徽省自然科学基金资助项目(11040606M97),安徽省优秀青年基金项目(08040106802)和安徽省水稻遗传育种重点试验室开放基金项目(SD2009-4)资助. （11040606M97）
