中国烟草学报2011，Vol.17Issue(4)：8-11,15,5.DOI:10.3969/j.issn.1004-5708.2011.04.002
高效阴离子色谱法测定卷烟主流烟气中的单糖
Analysis of monosaccharide in mainstream cigarette smoke by high performance anion exchange chromatography
吕健 1武超伟 2徐海涛 3周仕禄 2张峻松2
作者信息
- 1. 郑州轻工业学院食品与生物工程学院,郑州450002
- 2. 山东中烟工业有限责任公司技术中心,青岛266101
- 3. 河南中烟工业有限责任公司郑州卷烟厂,郑州450000
- 折叠
摘要
Abstract
Analytical method of 6 monosaccharides in cigarette mainstream smoke was developed using high performance anion-exchange chromatography (HPAEC) coupled with pulsed amperometric (amprerometric) detector. Total participate matter of mainstream cigarette smoke was collected on Cambridge filter pad and extracted in distilled water by ultrasonic bath. Separation was accomplished on a METROSEP Carbl (4.6 mm × 250 mm,5μm separation column by using elution consisting of solution of 2.0mmol/L NaOH and 0.5mmol/L NaAc as mobile phase at a flow rate of lmL /min. Under these conditions, fructose, glucose, arabinose, mannose, galactose, and xylose in TPM were separated. Sugars were detected with amprerometric detector. Excellent reproducibility was achieved with RSDs of 6 monosaccharides below 4.31% . Limit of detection for every monosaccharide compound was less than 0. lOfxg /cig. The recoveries were in the range of 85.10% to 95.52% .关键词
高效阴离子色谱/卷烟主流烟气/单糖/分析Key words
high performance anion exchange chromatography/ cigarette mainstream smoke/ monosaccharide/ analysis分类
轻工纺织引用本文复制引用
吕健,武超伟,徐海涛,周仕禄,张峻松..高效阴离子色谱法测定卷烟主流烟气中的单糖[J].中国烟草学报,2011,17(4):8-11,15,5.
