中国油料作物学报2011，Vol.33Issue(4)：384-390,7.
酸性土壤中大豆优势根瘤菌的分离、鉴定及其生物学特性
Isolation, identification and biological characteristics of Predominant rhizobia associated with soybean in acidic soil
摘要
Abstract
This paper studied molecular identification and biological characteristics of 29 stains rhizobia isolated and purified from soybean nodules grown on different acidic soils with different soybean cultivation histories at Yingtan, Jiangxi province and Jinhua, Zhejiang province. The nifH gene fragment were amplified from 16 strains of 29 isolates. Sequence analysis of nifH showed that these strains belonged to the genus of Bradyrhizobium. Ten rhi-zobial strains were from Jinhua and showed no obviously difference among soybean cultivars. Six rhizobial strains were from the nodules of BX10 (Al -tolerant) and BD2 (Al - sensitive) at Yingtan and showed differences between these two soybean genotypes. Based on the phylogenetic analysis of nifH sequence, the species of selected soybean rhizobia were confirmed by 16S rDNA sequence analysis and rhizobial inoculants. Four rhizobial strains showed nodules in the roots of BX10 and BD2, and the growth curve and physiological and biochemical characteristics of them were further identified. Results suggested that dominant rhizobia in acidic soils showed regional differences.关键词
大豆/根瘤菌/nifH/16S rDNA/生物学特性/酸性土壤Key words
Soybean/ Rhizobia/ nifH/ 16S rDNA/ Biological characteristics/ Acidic soil分类
农业科技引用本文复制引用
李永春,孔令如,王焱,孔文尧,杨统一,邹爱兰,戚金亮,杨永华..酸性土壤中大豆优势根瘤菌的分离、鉴定及其生物学特性[J].中国油料作物学报,2011,33(4):384-390,7.基金项目
国家自然科学基金项目(30971871,40371071) （30971871,40371071）
国家重大专项-转基因生物新品种培育重大专项子课题(2008ZX08011-003,2011ZX08011-002) （2008ZX08011-003,2011ZX08011-002）
