作物学报2011，Vol.37Issue(9)：1689-1694,6.DOI:10.3724/SP.J.1006.2011.01689
玉米杂交种先玉335及其亲本种子萌发过程中胚芽蛋白质组学分析
Proteomic Analysis of Plumule in Seed Germination for an Elite Hybrid Pioneer 335 and Its Parental Lines in Maize
摘要
Abstract
The plumule (including coleoptile) of seed cultured for 84 h was used as materials to analyze the proteomic difference between the elite hybrid Pioneer 335 and its parental lines by mean of 2-D electrophoresis method. The results showed that 560 protein spots were identified in Pioneer 335,507 and 508 protein spots parental lines in its PH6WC and PH4CV, respectively. And in Pioneer 335,81% protein spots exhibited nonadditive expression model. Out of them, 288 showed up-regulation with over high parent expression model. While only 15 showed down-regulation with over low parent expression model. Thus the accumulation of nonadditive expression protein might be the key factor resulting in heterosis at the tested development at stage in Pioneer 335 at least in this study. Notably, 13 significantly different protein spots between the hybrid and its parents were involved in metabolic pathway, protein folding, stress response, cytoskeleton and cell detoxification.关键词
玉米/胚芽(胚芽鞘)/杂种优势/蛋白质组学分析Key words
Maize/ Plumule (including cleotiple)/ Heterosis/ Proteomic analysis引用本文复制引用
进茜宁,付志远,丁冬,刘宗华,李卫华,汤继华..玉米杂交种先玉335及其亲本种子萌发过程中胚芽蛋白质组学分析[J].作物学报,2011,37(9):1689-1694,6.基金项目
本研究由国家高技术研究发展计划(863计划)重点项目(2009AA101103)和河南省重大公益项目资助. （863计划）
