四川师范大学学报（自然科学版）2011，Vol.34Issue(5)：706-709,4.DOI:10.3969/j.issn.1001-8395.2011.05.022
甲基百里酚蓝-钕(Ⅲ)配合物与DNA作用研究
The Interreaction of Methylthymol Blue-Nd(Ⅲ)Complexes with DNA
摘要
Abstract
The interaction of Methylthymol Blue(MTB)-Nd( Ⅲ) complexes with Herring Sperm DNA was investigated by UV-Vis spectrophotometric method in alkalescence buffer solution ( pH = 8. 25). The maximum absorption of formative Blue MTB-Nd( Ⅲ )-DNA coordination compound was obtained at 610 nm. The red shift of maximum absorption was 5 run compared to the MTB and MTB-DNA complex. DNA exists domino effect of augment colour to MTB-Nd ( Ⅲ ) . According to balance percolation mol ratio method and double wavelengh mol ratio method, working out the combine ratio n( MTB): n(Nd(Ⅲ)): n( DNA) is 10: 40: 1, combining con-stant of MTB-Nd(Ⅲ) complexe with DNA is K = S. 38 × 106.关键词
鲱鱼精DNA/甲基百里酚蓝(MTB)-钕(Ⅲ)-DNA复合物/UV-Vis光谱法Key words
methylthymol blue-Nd( Ⅲ) complexes/ methylthymol blue-Nd ( Ⅲ)-herring sperm DNA association compound/ UV-Vis spectra分类
化学化工引用本文复制引用
石荣铭,王兴明..甲基百里酚蓝-钕(Ⅲ)配合物与DNA作用研究[J].四川师范大学学报（自然科学版）,2011,34(5):706-709,4.基金项目
国家自然科学基金(30572254)资助项目 （30572254）
