中国油料作物学报2011，Vol.33Issue(4)：318-324,7.
甘蓝型油菜热激蛋白Bnp23相互作用蛋白的筛选和鉴定
Screening and identification of proteins interacting with Brassica napus heat shock protein 23
摘要
Abstract
Yeast two - hybrid assay was carried out to screen the interaction proteins of Brassica napus heat shock protein Bnp23 using activator domain - cDNA library of Arabidopsis thaliana seedlings. 39 positive clones were isolated; and among them ,2 clones were encoded by Arabidopsis PYK10 binding protein 1 (AtPBPl). In vitro interaction between AtPBPl and Bnp23 was further confirmed by western blotting. An interaction between AtPBPl homologous protein BnMBP6 (myrosase binding protein 6 of B. Napus) and Bnp23 was also identified by yeast two -hybrid assay.关键词
酵母双杂交/Bnp23/AtPBP1/油菜黑芥子酶结合蛋白Key words
Yeast two -hybrid/ Bnp23/ AtPBPl/ BnMBP6分类
生物科学引用本文复制引用
王芳,刘超,吴江生,张忠明..甘蓝型油菜热激蛋白Bnp23相互作用蛋白的筛选和鉴定[J].中国油料作物学报,2011,33(4):318-324,7.基金项目
教育部油菜遗传改良创新团队(IRT0442) （IRT0442）
华中农业大学自主科技创新基金青年教师科技创新专项(2011QC038) （2011QC038）
