同位素2011，Vol.24Issue(3)：188-192,5.
液质联用法测定常用18F药物中Kryptofix2.2.2的含量
Measurement of Kryptofix 2.2.2 in 18 F-radiopharmaceuticals by LC/MS/MS
摘要
Abstract
A high-performance liquid chromatography-tandem mass spectrometric method (LC/MS/MS) was used to quantify Kryptofix 2. 2. 2(K2. 2. 2) in 18F-FDG and 18F-FLT and other 18F-radiopharmaceuticals. First molecular ion peak m/z 377.4 [M+H]+ were detected by full scan mode, then the daughter ions 114.1, 289. 4 and 333. 5 m/z were detected by product ion scan mode, the mass scanning mode was set to multiple reaction monitoring (MRM) and programmed to monitor the transitions of the parent/daughter ion pairs of 377. 4/114. 1, 377. 4/289. 4 and 377. 4/333. 5 m/z for K2. 2. 2 . The calibration curve was established over the range of 20~500 μg/L, the linear correlation coefficient was 0. 999 9, the limit of quantitation for K2. 2. 2 was 20 μg/L. Reproducibility and accuracy all accorded with expectation. Direct measurement of the concentration of K2. 2. 2 in the routine radiopharma-ceutical of l8F-FDG was less than 20 μg/L, and the concentration of K2. 2. 2 in 5 batchs 18F-FLT products were between 0. 286 and 16. 9 mg/L. Above all, LC/MS/MS is the most sensitive method for the quantification of K2. 2. 2 so far.关键词
液质联用/18F药物/K2.2.2含量Key words
LC/MS/MS/ l8F-radiopharmaceuticals/ conceneration of Kryptofix 2. 2. 2分类
医药卫生引用本文复制引用
张晓军,李云刚,刘健,张锦明,田嘉禾..液质联用法测定常用18F药物中Kryptofix2.2.2的含量[J].同位素,2011,24(3):188-192,5.基金项目
十一五国家支撑计划(2008BA155B00) （2008BA155B00）
