实验动物与比较医学2011，Vol.31Issue(5)：376-380,5.DOI:10.3969/j.issn.1674-5817.2011.05.016
两个品系小鼠肺纤维化模型的比较观察
Comparative Observation on Pulmonary Fibrosis Models between Two Strains of Mice
刘蓉 1赵瑾 1李洪涛 1谭亚夏 1陈国勤2
作者信息
- 1. 广州医学院第一附属医院,呼吸疾病国家重点实验室,广州510120
- 2. 广州医学院第一附属医院病理科,广州510120
- 折叠
摘要
Abstract
Objective To provide a better animal model of pulmonary fibrosis by comparing the model establishment in different strains of mice. Methods BALB/c and C57BL/6 mice were divided into model and control group respectively, which received intratracheal injection of bleomycin (BLM) and normal saline, respectively. The mice were sacrificed 28 days after treatment. The masson mass and the degree of fibrosis in the left pulmonary lobe were observed by light microscope. Hydroxyproline contents in right pulmonary lobe were measured. Results The pulmonary consolidation and collagen deposition appeared in all bleomycin groups especially in the C57BL/6 group. Hydroxyproline contents in pulmonary tissue in C57BL/6 mice was the highest and normal saline group was the lowest one. Conclusion The pulmonary fibrosis model can be established in BALB/c and C57BL/6 mice by intratracheal injection of bleomycin, while the C57BL/6 mice was more suitable.关键词
小鼠品系/肺纤维化/动物模型/博莱霉素Key words
Strains of mice/ Pulmonary fibrosis/ Animal model/ Bleomycin分类
医药卫生引用本文复制引用
刘蓉,赵瑾,李洪涛,谭亚夏,陈国勤..两个品系小鼠肺纤维化模型的比较观察[J].实验动物与比较医学,2011,31(5):376-380,5.
