实验动物与比较医学2011，Vol.31Issue(5)：338-344,7.DOI:10.3969/j.issn.1674-5817.2011.05.007
蚜肠霉素处理及细胞传代数对小型猪体细胞核移植胚胎发育的影响
Effects of Different Donor Cells Treatment with Aphidicolin and Donor Cell Passages on the Preimplantation Development of Somatic Cell Nuclear Transfer Embryos in Minipig
摘要
Abstract
Objective To optimize somatic cell nuclear transfer (SCNT) procedures in mini-pigs, the present study was designed to examine the effects of donor cell types, cell passage number and aphidicolin (APC) treatment on in vitro development of reconstructed embryos. Methods The Oviduct epithelial cells (OEC), ear fibroblast cells (EFC) and cumulus cells (CC) derived from mini-pigs were treated with serum starvation only or serum starvation followed by treatment of 0.1 ^g/ml APC. The reconstructed embryos were cultured for 7 days to evaluate their developmental competency. Results Cleavage and blastocyst formation rates of reconstructed embryos derived from the OEC by APC treatment were significantly higher than the serum starvation (61.82% vs 56.25%, 24.55% vs 17.86%; P＜0.05). The cleavage rate from the EFC was significantly increased by APC treatment compared to serum starvation only (63.36% vs 57.01%; P＜0.05). In the ooctyes with the CC, the reconstructed embryos could yield high blastocysts formation rate by APC treatment (29.63%; P＜0.05). In the presence of APC, CC gave rise to highest cleavage and blastocyst formation rates among the three cell types. All of the three donors can be used as donor karyoplasts for nuclear transfer, and the efficiency is not influenced by culture passages. Conclusion The treatment of CC with serum starvation plus APC prior to nuclear transfer is more suitable in SCNT of mini-pigs.关键词
Aphidicolin/小型猪/细胞类型/传代数/体细胞核移植Key words
aphidicolin/ minipig/ cell type/ passage number/ somatic cell nuclear transfer.分类
生物科学引用本文复制引用
张廷宇,戴建军,张树山,吴彩凤,张德福..蚜肠霉素处理及细胞传代数对小型猪体细胞核移植胚胎发育的影响[J].实验动物与比较医学,2011,31(5):338-344,7.基金项目
国家转基冈生物新品种培育重大专项(2009ZX08006-014B （）
2008ZX08006-005) （）
上海市科技兴农推广项目(2007-3-7) （2007-3-7）
上海市农业科学院青年基金(QJ2011-07) （QJ2011-07）
