南京理工大学学报（自然科学版）2011，Vol.35Issue(4)：558-562,5.
基因重组酵母工程菌优化表达人源胶原蛋白
Optimization of Fermentation Conditions of Genetically Engineered Pichia pastoris Expressing Recombinant Human-source Collagen
摘要
Abstract
Response surface methodology (RSM) is used to optimize the fermentation conditions of genetically engineered Pichia pastoris( GS115/pPIC9KG6) expressing highly hydrophilic recombinant human-source collagen. Box-Behnken design ( BBD)is introduced to estimate the impact of initial inducement pH, temperature and addition amount of methanol. An experiment is done by setting different culture conditions according to the scheme designed through the DX7Trial software,and the correlational parameters are determined. The RSM analysis result shows that the optimal scale of factors is:initial inducement pH 5.0,temperature 28℃,addition amount of methanol 2. 2%/24 h. The experiment offers precise conditions for high density fermentation performed in fermenter.关键词
巴氏毕赤酵母/重组人源胶原蛋白/响应曲面法/Box-Behnken设计Key words
Pichia pastoris/ recombinant human-source collagen/ response surface methodology/ Box-Behnken design分类
生物科学引用本文复制引用
刘斌,郭红丽,钮小松,张小霞,杨树林..基因重组酵母工程菌优化表达人源胶原蛋白[J].南京理工大学学报（自然科学版）,2011,35(4):558-562,5.基金项目
国家“863”计划资助项目(2008AA12A218) （2008AA12A218）
教育部博士点基金(20093219110013) （20093219110013）
