中国水产科学2011，Vol.18Issue(5)：992-999,8.DOI:10.3724/SP.J.1118.2011.00992
鳜胃蛋白酶基因外显子7上SNP检测及其与食性驯化相关分析
Association of polymorphism detection of SNPs in exon 7 of pepsinogen (PEP) and feeding behavior domestication in Siniperca chuatsi
摘要
Abstract
Sinperca chuatsi refuse to eat dead prey or man-made feed. However, experiments with long-term cultivation suggest that S. Chuatsi can be domesticated to feed on inert baits. Selective breeding of S. Chuatsi using molecular markers and mass cultivation with artificial feed reduces the problems associated with cost, contamination, and disease. Pepsinogen is an acid gaster-digestion hydrolase which digests proteins under acidic conditions. Acidic proteases are sometimes used as additives in animal feed as they promote digestion and absorption of nutrients in fish. In order to search the distribution of the alleles and genotypes of pepsin gene (PEP) gene between domesticated and undomesticated populations, we identified SNPs in introns S, 6, 7, and 8 and exons 6, 7, and 8 of the PEP in S. Chuatsi using DS, PCR-RFLP, and CRS-PCR. Two SNP sites (TIC, C52T) was identified in exon 7 of the PEP gene, both of which were synonymous mutations. There was not significant difference in the occurrence of the SNPs between domesticated and undomesticated populations (P>0.05). Five diplotypes were constructed based on two SNPs in the experimental population. There was a significant association between diplo-typel and diplotypeS in the two populations (P<0.05). Our results provide a foundation for marker assisted selective breeding. The PEP gene offers considerable potential as a candidate marker for the domesticated feeding phenotype in S. Chuatsi.关键词
鳜/胃蛋白酶/SNP/食性驯化Key words
Siniperca chuatsi/ pepsinogen/ single nucleotide polymorphism/ feeding behavior domestication分类
农业科技引用本文复制引用
方荣,梁旭方,杨宇晖,杜嵇华,曹亮,叶卫,符云..鳜胃蛋白酶基因外显子7上SNP检测及其与食性驯化相关分析[J].中国水产科学,2011,18(5):992-999,8.基金项目
广东省海洋渔业科技推广专项项目(A200899D02) （A200899D02）
广州市番禺区科技计划项目(2009-Z-73-1) （2009-Z-73-1）
广州市科技计划项目(2009Z1-E711). （2009Z1-E711）
